منابع مشابه
Preparation and Expression of M2 Gene Plasmid DNA for Potential use in Influenza A Vaccine Production
No abstract this article: Preparation and Expression of M2 Gene Plasmid DNA for Potential use in Influenza A Vaccine Production
متن کاملPhysical Factors Affecting Plasmid DNA Compaction in Stearylamine-Containing Nanoemulsions Intended for Gene Delivery
Cationic lipids have been used in the development of non-viral gene delivery systems as lipoplexes. Stearylamine, a cationic lipid that presents a primary amine group when in solution, is able to compact genetic material by electrostatic interactions. In dispersed systems such as nanoemulsions this lipid anchors on the oil/water interface confering a positive charge to them. The aim of this wor...
متن کاملThe arrival of genetic engineering: strategies for delivery of nonviral plasmid DNA-based gene therapy
for electronic indexing) The delivery of a therapeutic gene inserted in a vector for somatic gene therapy holds great promise for the future. Encouraging results from the clinical trials of first-generation plasmid-based medicines/ vaccines have further validated the importance of molecular biology and biotechnology research. With newer developments in bioengineering for the mass production and...
متن کاملTernary complex of plasmid DNA with protamine and γ-polyglutamic acid for biocompatible gene delivery system.
The purpose of the present study was to investigate the usefulness of the ternary complex with protamine and γ-polyglutamic acid (γ-PGA), which are biodegradable materials for foods and medical products, as a safe gene delivery vector. We formed cationic binary complexes (plasmid DNA (pDNA)/protamine complexes) with high transfection efficiency. The binary complex showed slight toxicity probabl...
متن کاملRational vector design for efficient non-viral gene delivery: challenges facing the use of plasmid DNA.
Although non-viral gene delivery is a very straightforward technology, there are currently no FDA-approved gene medicinal products available. Therefore, improving potency, safety, and efficiency of current plasmid DNA vectors will be a major task for the near future. This article will provide an overview on factors influencing production yield and quality as well as safety issues that emerge fr...
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ژورنال
عنوان ژورنال: Materials Today
سال: 2004
ISSN: 1369-7021
DOI: 10.1016/s1369-7021(04)00612-1